497 Protein biomarkers in the paraffine-archived human melanoma samples

Since there are only a few tissue markers used in the routine histopathology of melanoma, is an unmet need for novel protein discovery both prognostic and predictive implications. Our introduction formalin-fixed paraffin-embedded (FFPE) melanoma samples provides new opportunities to understand progression melanoma. The study was based on previously archived 175 human FFPE primary metastatic which were annotated by enhanced pathology validation mutated BRAF analyzed high-resolution mass spectrometry. gained results correlated with clinicopathologic (survival therapy response) data patients. Protein expressions formed different sample clusters due enrichment shorter longer survival. Regarding prediction immunotherapy response, VEGFA-VEGFR2, RNA splicing, immunoregulation pathways patient outcome. metabolic extracellular matrix regulation have also been proven as significant actors inhibition. heterogeneity mutation detection through comparison immunohistochemistry DNA sequencing data, well expression may create opportunity personalized medicine translational purposes, highlighting importance paraffin-archived material during